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Creators/Authors contains: "Cassat, James E."

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  1. ; ; ; ; ; ( , Analytical Chemistry)
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  2. ; ; ; ; ; ; ( , Current Protocols)
    Abstract

    Staphylococcus aureusis a Gram‐positive bacterium that colonizes almost every organ in humans and mice and is a leading cause of diseases worldwide.S. aureusinfections can be challenging to treat due to widespread antibiotic resistance and their ability to cause tissue damage. The primary modes of transmission ofS. aureusare via direct contact with a colonized or infected individual or invasive spread from a colonization niche in the same individual.S. aureuscan cause a myriad of diseases, including skin and soft tissue infections (SSTIs), osteomyelitis, pneumonia, endocarditis, and sepsis.S. aureusinfection is characterized by the formation of purulent lesions known as abscesses, which are rich in live and dead neutrophils, macrophages, and surrounded by a capsule containing fibrin and collagen. Different strains ofS. aureusproduce varying amounts of toxins that evade and/or elicit immune responses. Therefore, animal models ofS. aureusinfection provide a unique opportunity to understand the dynamics of organ‐specific immune responses and modifications in the pathogen that could favor the establishment of the pathogen. With advances in in vivo imaging of fluorescent transgenic mice, combined with fluorescent/bioluminescent bacteria, we can use mouse models to better understand the immune response to these types of infections. By understanding the host and bacterial dynamics within various organ systems, we can develop therapeutics to eliminate these pathogens. This module describes in vivo mouse models of both local and systemicS. aureusinfection. © 2021 Wiley Periodicals LLC.

    This article was corrected on 20 July 2022. See the end of the full text for details.

    Basic Protocol 1: Murine model ofStaphylococcus aureussubcutaneous infection

    Alternate Protocol: Murine tape stripping skin infection model

    Basic Protocol 2: Sample collection to determine skin structure, production of inflammatory mediators, and bacterial load

    Basic Protocol 3: Murine model of post‐traumaticStaphylococcus aureusosteomyelitis

    Basic Protocol 4: Intravenous infection of the retro‐orbital sinus

    Support Protocol: Preparation of the bacterial inoculum

     
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